Clostridium difficile R20291

View alignment of region with nucmer promer

Design primers within region using Primer3Plus

View region in Artemis

Retrieve CDR20291_0004 nucleotide sequence in FASTA format

Retrieve CDR20291_0004 protein sequence in FASTA format

Retrieve CDR20291_0004 nucleotide sequence with 10 50 100 200 400 600 800 1000 2000 bp. flanking sequence
Include translation of coding sequence
Include complementary strand.

Run NCBI BLASTP PSI-BLAST against the Non-redundant protein sequences (nr) SwissProt Protein Data Bank (PDB) database using CDR20291_0004 protein sequence
(optional - restrict search to taxonomic group: root cellular organisms Bacteria Firmicutes Clostridia Clostridiales Clostridiaceae Clostridium Clostridium difficile )

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