Clostridium difficile R20291

View alignment of region with nucmer promer

Design primers within region using Primer3Plus

View region in Artemis

Retrieve sigB nucleotide sequence in FASTA format

Retrieve sigB protein sequence in FASTA format

Retrieve CDR20291_3552 nucleotide sequence with 10 50 100 200 400 600 800 1000 2000 bp. flanking sequence
Include translation of coding sequence
Include complementary strand.

Run NCBI BLASTP PSI-BLAST against the Non-redundant protein sequences (nr) SwissProt Protein Data Bank (PDB) database using CDR20291_3552 protein sequence
(optional - restrict search to taxonomic group: root cellular organisms Bacteria Firmicutes Clostridia Clostridiales Clostridiaceae Clostridium Clostridium difficile )

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