Escherichia coli O103:H2 str. 12009

View alignment of region with nucmer promer

Design primers within region using Primer3Plus

View region in Artemis

Retrieve carB nucleotide sequence in FASTA format

Retrieve carB protein sequence in FASTA format

Retrieve ECO103_0034 nucleotide sequence with 10 50 100 200 400 600 800 1000 2000 bp. flanking sequence
Include translation of coding sequence
Include complementary strand.

Run NCBI BLASTP PSI-BLAST against the Non-redundant protein sequences (nr) SwissProt Protein Data Bank (PDB) database using ECO103_0034 protein sequence
(optional - restrict search to taxonomic group: root cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli O103:H2 )

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