Escherichia coli str. K-12 substr. DH10B

View alignment of region with nucmer promer

Design primers within region using Primer3Plus

View region in Artemis

Retrieve insA-1 nucleotide sequence in FASTA format

Retrieve insA-1 protein sequence in FASTA format

Retrieve ECDH10B_0023 nucleotide sequence with 10 50 100 200 400 600 800 1000 2000 bp. flanking sequence
Include translation of coding sequence
Include complementary strand.

Run NCBI BLASTP PSI-BLAST against the Non-redundant protein sequences (nr) SwissProt Protein Data Bank (PDB) database using ECDH10B_0023 protein sequence
(optional - restrict search to taxonomic group: root cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli K-12 )

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